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AFP-L3 (Shorten Name),
Product Name:

Human AFP-L3/ Alpha-fetoprotein Lens culinaris agglutiin 3 ELISA Kit

 
Cat.#:

E1117h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.624-40ng/mL
Sensitivity:
0.312ng/mL
Specificity:
Natural and recombinant human Alpha-fetoprotein Lens culinaris agglutiin 3
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1117h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Alpha-fetoprotein Lens culinaris agglutiin 3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Alpha-fetoprotein Lens culinaris agglutiin 3. The ELISA analytical biochemical technique of the E1117h kit is based on Alpha-fetoprotein Lens culinaris agglutiin 3 antibody-Alpha-fetoprotein Lens culinaris agglutiin 3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Alpha-fetoprotein Lens culinaris agglutiin 3 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Alpha-fetoprotein Lens culinaris agglutiin 3. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human AFP-L3 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-fetoprotein Lens culinaris agglutiin 3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-89%

105-113%

82-92%

100-113%

EDTA plasma(n=5)

106-116%

92-102%

95-107%

104-116%

heparin plasma(n=5)

94-105%

 

102-112%

112-112%

80-93%

 

General Annotation


Sub Unit:
N/A


Function:
In oncology, AFP-L3 is an isoform of Alpha-fetoprotein (AFP), a substance typically used in the triple test during pregnancy and for screening chronic liver disease patients for hepatocellular carcinoma (HCC). AFP can be fractionated by affinity electrophoresis into 3 glycoforms: L1, L2, and L3 based on the reactivity with the lectin Lens culinaris agglutinin (LCA). AFP-L3 binds strongly to LCA via an additional α 1-6 fucose residue attached at the reducing terminus of N-acetylglucosamine; this is in contrast to the L1 isoform. It is the L1 isoform which is typically associated with non-HCC inflammation of liver disease condition. The L3 isoform is specific to malignant tumors and its detected presence can serve to identify patients whom need increased monitoring for the development of HCC in high risk populations (i.e. chronic hepatitis B & C and/or liver cirrhosis). AFP-L3% is now being considered as a tumor marker for the North American demographic.


Location:
N/A


Sample Data
Sample Data
Sample Data
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