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Beta-APP42 (Shorten Name),
Product Name:

Human Beta-APP42/ Beta-amyloid protein 42 ELISA Kit

 
Cat.#:

E1151h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500pg/mL
Sensitivity:
3.12 pg/mL
Specificity:
Natural and recombinant human Beta-amyloid protein 42
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1151h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Beta-amyloid protein 42) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Beta-amyloid protein 42. The ELISA analytical biochemical technique of the E1151h kit is based on Beta-amyloid protein 42 antibody-Beta-amyloid protein 42 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Beta-amyloid protein 42 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Beta-amyloid protein 42. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human Beta-APP42 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9%

Recovery
Recovery was determined by spiking various levels of Beta-amyloid protein 42 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-amyloid protein 42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-102%

92-105%

110-120%

80-92%

EDTA plasma(n=5)

108-118%

94-102%

94-104%

100-109%

heparin plasma(n=5)

82-92%

 

81-90%

114-124%

101-110%

 

General Annotation


Sub Unit:
N/A


Function:
Human form of the predominant amyloid β-peptide found in plaques associated with Alzheimer's disease. Leads to a large shift in the bax/bcl-2 ratio in favour of pro-apoptotic bax. Neurotoxic.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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