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DPD (Shorten Name),
Product Name:

General DPD/ Deoxypyridinoline ELISA Kit

 
Cat.#:

E0894Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100ng/mL
Sensitivity:
0.47ng/mL
Specificity:
Natural and recombinant general Deoxypyridinoline
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0894Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Deoxypyridinoline) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Deoxypyridinoline. The ELISA analytical biochemical technique of the E0894Ge kit is based on Deoxypyridinoline antibody-Deoxypyridinoline antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Deoxypyridinoline antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Deoxypyridinoline. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General DPD ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Deoxypyridinoline and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-110%

108-118%

89-99%

102-112%

EDTA plasma(n=5)

101-111%

93-104%

92-103%

86-94%

heparin plasma(n=5)

94-102%

 

107-117%

97-107%

103-112%

 

General Annotation


Sub Unit:
N/A


Function:
Deoxypyridinoline, also called D-Pyrilinks, Pyrilinks-D, or deoxyPYD, is one of two pyridinium cross-links that provide structural stiffness to type I collagen found in bones. It is excreted unmetabolized in urine and is a specific marker of bone resorption and osteoclastic activity. It is measured in urine tests and is used along with other bone markers such as alkaline phosphatase, osteocalcin, and N-terminal telopeptide to diagnose bone diseases such as postmenopausal osteoporosis, bone metastasis, and Paget's disease, furthermore, it has been useful in monitoring treatments that conatin bone-active agents such as estrogens and bisphosphonates.


Location:
N/A


Sample Data
Sample Data
Sample Data
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