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HVA (Shorten Name),
Product Name:

General HVA/ Homovanillic acid ELISA Kit

 
Cat.#:

E1381Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.29ng/mL
Specificity:
Natural and recombinant general Homovanillic acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1381Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Homovanillic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Homovanillic acid. The ELISA analytical biochemical technique of the E1381Ge kit is based on Homovanillic acid antibody-Homovanillic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Homovanillic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Homovanillic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General HVA ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Homovanillic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-115%

105-115%

84-94%

106-118%

EDTA plasma(n=5)

96-106%

103-113%

103-113%

104-116%

heparin plasma(n=5)

105-115%

 

92-102%

94-103%

102-112%

 

General Annotation


Sub Unit:
N/A


Function:
Homovanillic acid (HOC6H3(OCH3)CH2COOH; synonyms: 3-Methoxy-4-hydroxyphenyl acetic acid; HVA; 4-Hydroxy-3-methoxy-benzeneacetic acid; 4-Hydroxy-3-methoxyphenylacetic acid) is a major catecholamine metabolite. It is used as a reagent to detect oxidative enzymes, and is associated with dopamine levels in the brain. In psychiatry and neuroscience, brain and cerebrospinal fluid levels of HVA are measured as a marker of metabolic stress caused by 2-deoxy-D-glucose. HVA presence supports a diagnosis of neuroblastoma and malignant pheochromocytoma. Fasting plasma levels of HVA are known to be higher in females than in males. This does not seem to be influenced by adult hormonal changes, as the pattern is retained in the elderly and post-menopausal as well as transsexuals according to their genetic sex, both before and during cross-sex hormone administration. Differences in HVA have also been correlated to tobacco usage, with smokers showing significantly lower amounts of plasma HVA.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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