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Home  >  ELISA Kit  >  Human CA50 ELISA Kit
CA50 (Shorten Name ),
Product Name:

Human CA50/ Carbohydrate Antigen 50 ELISA Kit

Cat.#:

E1221h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.79 ng/mL
Specificity:
Natural and recombinant human Carbohydrate Antigen 50
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human CA50 ELISA Kit
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Human CA50 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of Carbohydrate Antigen 50 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbohydrate Antigen 50 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

104-114%

94-104%

107-117%

EDTA plasma(n=5)

104-104%

108-119%

108-117%

90-101%

heparin plasma(n=5)

81-91%

 

100-110%

116-128%

96-106%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


This product has not yet been referenced specifically in any publications.
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