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CA50 (Shorten Name),
Product Name:

Human CA50/ Carbohydrate Antigen 50 ELISA Kit

 
Cat.#:

E1221h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.79 ng/mL
Specificity:
Natural and recombinant human Carbohydrate Antigen 50
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1221h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Carbohydrate Antigen 50) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Carbohydrate Antigen 50. The ELISA analytical biochemical technique of the E1221h kit is based on Carbohydrate Antigen 50 antibody-Carbohydrate Antigen 50 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Carbohydrate Antigen 50 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Carbohydrate Antigen 50. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human CA50 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of Carbohydrate Antigen 50 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbohydrate Antigen 50 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-107%

104-114%

94-104%

107-117%

EDTA plasma(n=5)

104-104%

108-119%

108-117%

90-101%

heparin plasma(n=5)

81-91%

 

100-110%

116-128%

96-106%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


This product has not yet been referenced specifically in any publications.
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