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NT-proBNP (Shorten Name ),
Product Name:

Rat NT-proBNP ELISA Kit

Cat.#:

E0485r

Brand:
EIAab®
Regulatory Status:
Alternative:

N-terminal prohormone of brain natriuretic peptide

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/ml
Sensitivity:
7.86pg/mL
Specificity:
Natural and recombinant rat NT-proBNP
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat NT-proBNP ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.9%

Recovery
Recovery was determined by spiking various levels of NT-proBNP into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-proBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

114-123%

99-108%

106-114%

EDTA plasma(n=5)

110-120%

91-101%

107-115%

110-120%

heparin plasma(n=5)

104-115%

 

108-118%

97-107%

108-116%

 

General Annotation


Sub Unit:
N/A


Function:
The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a N-terminal fragment of brain natriuretic peptide.


Location:
Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome. The plasma concentrations of both BNP and NT-proBNP are also typically increased in patients with asymptomatic or symptomatic left ventricular dysfunction.


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