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MMA (Shorten Name),
Product Name:

General MMA/ Methylmalonic Acid ELISA Kit

 
Cat.#:

E2091Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.099ng/mL
Specificity:
Natural and recombinant general Methylmalonic Acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E2091Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Methylmalonic Acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Methylmalonic Acid. The ELISA analytical biochemical technique of the E2091Ge kit is based on Methylmalonic Acid antibody-Methylmalonic Acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Methylmalonic Acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Methylmalonic Acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General MMA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Methylmalonic Acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-112%

81-91%

102-111%

111-120%

EDTA plasma(n=5)

97-110%

102-113%

105-115%

86-96%

heparin plasma(n=5)

105-116%

 

110-120%

104-115%

105-115%

 

General Annotation


Sub Unit:
N/A


Function:
Methylmalonic acid (MMA) is a dicarboxylic acid that is a C-methylated derivative of malonate. The coenzyme A linked form of methylmalonic acid, methylmalonyl-CoA, is converted into succinyl-CoA by methylmalonyl-CoA mutase, in a reaction that requires vitamin B12 as a cofactor. In this way, it enters the Krebs cycle, and is thus part of one of the anaplerotic reactions.


Location:
N/A


Sample Data
Sample Data
Sample Data
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