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PAFA (Shorten Name),
Product Name:

General PAFA/ Platelet-activating factor ELISA Kit

 
Cat.#:

E0526Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.09ng/mL
Specificity:
Natural and recombinant general Platelet-activating factor
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0526Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Platelet-activating factor) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Platelet-activating factor. The ELISA analytical biochemical technique of the E0526Ge kit is based on Platelet-activating factor antibody-Platelet-activating factor antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Platelet-activating factor antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Platelet-activating factor. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General PAFA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet-activating factor and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-112%

104-113%

99-109%

84-94%

EDTA plasma(n=5)

94-106%

111-120%

95-103%

100-110%

heparin plasma(n=5)

100-111%

 

108-119%

102-111%

100-113%

 

General Annotation


Sub Unit:
N/A


Function:
It is an important mediator of bronchoconstriction. It causes platelets to aggregate and blood vessels to dilate. Thus, it is important to the process of hemostasis. At a concentration of 1E-12 mol/L, PAF causes life threatening inflammation of the airways to induce asthma like symptoms. Toxins such as fragments of destroyed bacteria induce the synthesis of PAF, which causes a drop in blood pressure and reduced volume of blood pumped by the heart, which leads to shock and possibly death.


Location:
Platelet-activating factor, also known as a PAF, PAF-acether or AGEPC (acetyl-glyceryl-ether-phosphorylcholine) is a potent phospholipid activator and mediator of many leukocyte functions, including platelet aggregation and degranulation, inflammation, and anaphylaxis. It is also involved in changes to vascular permeability, the oxidative burst, chemotaxis of leukocytes, as well as augmentation of arachidonic acid metabolism in phagocytes. It is produced in response to specific stimuli by a variety of cell types, including neutrophils, basophils, injured tissue, monocytes/macrophages, platelets, and endothelial cells.


Sample Data
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Sample Data
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