027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human CT ELISA Kit
CT (Shorten Name),
Product Name:

Human CT/ Calcitonin ELISA Kit

 
Cat.#:

E0472h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
12.5-800pg/mL
Sensitivity:
4.5pg/mL
Specificity:
Natural and recombinant human Calcitonin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Signal Transduction
Product Overview:
E0472h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Calcitonin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Calcitonin. The ELISA analytical biochemical technique of the E0472h kit is based on Calcitonin antibody-Calcitonin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Calcitonin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Calcitonin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
Human CT ELISA Kit
Package & Price: Sign in.
Attention Attention
×
Human CT ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

Recovery
Recovery was determined by spiking various levels of Calcitonin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

111

105-117

Plasma

113

107-119

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

88-100%

103-115%

108-116%

97-108%

EDTA plasma(n=5)

94-103%

89-99%

83-93%

109-120%

heparin plasma(n=5)

107-115%

 

86-97%

104-114%

92-101%

 

General Annotation


Sub Unit:
N/A


Function:
Calcitonin (also known as thyrocalcitonin) is a 32-amino acid linear polypeptide hormone that is produced in humans primarily by the parafollicular cells (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchial body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone (PTH).


Location:
Secreted


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com