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PCT (Shorten Name),
Product Name:

Human PCT/ Procalcitonin ELISA Kit

 
Cat.#:

E0689h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/mL
Sensitivity:
3.9pg/mL
Specificity:
Natural and recombinant human Procalcitonin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Signal Transduction
Product Overview:
E0689h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Procalcitonin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Procalcitonin. The ELISA analytical biochemical technique of the E0689h kit is based on Procalcitonin antibody-Procalcitonin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Procalcitonin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Procalcitonin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human PCT ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of Procalcitonin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Procalcitonin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

112-122%

90-100%

93-103%

105-117%

EDTA plasma(n=5)

103-112%

106-116%

81-92%

99-108%

heparin plasma(n=5)

83-96%

 

105-114%

88-98%

98-109%

 

General Annotation


Sub Unit:
N/A


Function:
The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (10 pg/mL) of clinical assays. The level of procalcitonin raises in a response to a proinflammatory stimulus, especially of bacterial origin. In this case, it is produced mainly by the cells of the lung and the intestine. It does not raise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 ng/ml. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or serum calcium levels.


Location:
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine.


Sample Data
Sample Data
Sample Data
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