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Oxtc (Shorten Name),
Product Name:

General Oxtc/ Oxytocin ELISA Kit

 
Cat.#:

E9802Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
31.2-2000pg/mL
Sensitivity:
9.4pg/mL
Specificity:
Natural and recombinant general Oxytocin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E9802Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Oxytocin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Oxytocin. The ELISA analytical biochemical technique of the E9802Ge kit is based on Oxytocin antibody-Oxytocin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Oxytocin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Oxytocin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General Oxtc ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Oxytocin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

109-117%

105-114%

95-105%

91-91%

EDTA plasma(n=5)

81-93%

92-92%

80-90%

92-103%

heparin plasma(n=5)

96-105%

 

110-120%

109-117%

106-115%

 

General Annotation


Sub Unit:
N/A


Function:
Oxytocin is a mammalian hormone that acts primarily as a neuromodulator in the brain. Oxytocin is best known for its roles in sexual reproduction, in particular during and after childbirth. It is released in large amounts after distension of the cervix and uterus during labor, facilitating birth, and after stimulation of the nipples, facilitating breastfeeding.


Location:
Oxytocin is a peptide of nine amino acids (a nonapeptide). Its systematic name is cysteine-tyrosine-isoleucine-glutamine-asparagine-cysteine-proline-leucine-glycine-amine (cys – tyr – ile – gln – asn – cys – pro – leu – gly - NH2, or CYIQNCPLG-NH2). The cysteine residues form a disulfide bond. Oxytocin has a molecular mass of 1007 daltons. One international unit (IU) of oxytocin is the equivalent of about 2 micrograms of pure peptide.


Sample Data
Sample Data
Sample Data
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