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IgG (Shorten Name ),
Product Name:

Mouse IgG/ Immunoglobulin G ELISA Kit

Cat.#:

E0544m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.625-40ng/ml
Sensitivity:
0.32ng/mL
Specificity:
Natural and recombinant mouse Immunoglobulin G
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Mouse IgG ELISA Kit
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Mouse IgG ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin G into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

81

80-87

Plasma

83

80-89

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

84-96%

97-106%

108-118%

101-114%

EDTA plasma(n=5)

88-100%

84-95%

111-119%

103-114%

heparin plasma(n=5)

96-106%

 

108-119%

101-111%

91-103%

 

General Annotation


Sub Unit:
N/A


Function:
IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response.


Location:
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains — two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells.


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