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ProHAMP (Shorten Name),
Product Name:

Human ProHAMP/ Hepcidin prohormone ELISA Kit

 
Cat.#:

E1995h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.078ng/mL
Specificity:
Natural and recombinant human Hepcidin prohormone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1995h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Hepcidin prohormone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hepcidin prohormone. The ELISA analytical biochemical technique of the E1995h kit is based on Hepcidin prohormone antibody-Hepcidin prohormone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hepcidin prohormone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Hepcidin prohormone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human ProHAMP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Recovery
Recovery was determined by spiking various levels of Hepcidin prohormone into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

88

82-94

Plasma

90

84-96

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepcidin prohormone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

82-92%

89-98%

84-94%

111-120%

EDTA plasma(n=5)

100-110%

111-120%

96-106%

97-97%

heparin plasma(n=5)

107-118%

 

106-114%

95-104%

102-114%

 

General Annotation


Sub Unit:
N/A


Function:
Hepcidin preprohormone, prohormone, and hormone size are 84, 60, and 25 amino acids, respectively. Twenty- and 22-amino acid forms of Hepcidin also exist in the urine. The N terminal region is required for function, and deletion of 5 N-terminal peptides results in a loss of function. The conversion of prohepcidin to hepcidin is mediated by the prohormone convertase furin, which conversion might be regulated by the alpha-1 antitrypsin.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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