General NA/ Noradrenaline ELISA Kit

Cat.#:

E0907Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

NE,Norepinephrine,Isoprenaline

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

31.2-2000pg/mL

Sensitivity:

9.8pg/mL

Specificity:

Natural and recombinant general Noradrenaline

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0907Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Noradrenaline) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Noradrenaline. The ELISA analytical biochemical technique of the E0907Ge kit is based on Noradrenaline antibody-Noradrenaline antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Noradrenaline antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Noradrenaline. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General NA ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 2 )
Sequence / 3D
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Noradrenaline and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	82-92%	80-90%	86-96%	98-108%
EDTA plasma(n=5)	90-103%	110-118%	98-110%	84-96%
heparin plasma(n=5)	84-94%	87-97%	92-101%	104-114%

General Annotation

Sub Unit:

N/A

Function:

One of the most important functions of Noradrenaline is its role as the neurotransmitter released from the sympathetic neurons affecting the heart. An increase in Noradrenaline from the sympathetic nervous system increases the rate of contractions. When Noradrenaline acts as a drug, it increases blood pressure by increasing vascular tone (tension of vascular smooth muscle) through α-adrenergic receptor activation; a reflex bradycardia homeostatic baroreflex is overcome by a compensatory reflex preventing an otherwise inevitable drop in heart rate to maintain blood pressure.

Location:

Noradrenaline is synthesized from dopamine by dopamine β-hydroxylase.[8] It is released from the adrenal medulla into the blood as a hormone, and is also a neurotransmitter in the central nervous system and sympathetic nervous system, where it is released from noradrenergic neurons in the locus coeruleus. The actions of norepinephrine are carried out via the binding to adrenergic receptors.

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