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INTX (Shorten Name),
Product Name:

Human INTX/ Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain ELISA Kit

 
Cat.#:

E0639h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.15ng/mL
Specificity:
Natural and recombinant human Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0639h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain. The ELISA analytical biochemical technique of the E0639h kit is based on Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain antibody-Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human INTX ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross-linked N-terminal telopeptide of Collagen alpha-1(I) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-112%

110-120%

116-116%

86-96%

EDTA plasma(n=5)

91-103%

106-116%

98-108%

101-111%

heparin plasma(n=5)

82-90%

 

93-102%

105-115%

83-93%

 

General Annotation


Sub Unit:
N/A


Function:
In bone physiology, the N-terminal telopeptide (or more formally, amino-terminal collagen crosslinks, and known by the acronym NTX) is a biomarker used to measure the rate of bone turnover. NTX can be measured in the urine or serum.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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