25OHVD (Shorten Name),
Product Name:
General 25OHVD/ 25-OH Vitamin D ELISA Kit
Cat.#:
E0915Ge
Brand:
EIAab®
Regulatory Status:
Alternative:
Calcifediol,Calcidiol
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200nmol/L
Sensitivity:
1.27nmol/L
Specificity:
Natural and recombinant general 25-OH Vitamin D
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0915Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (25-OH Vitamin D) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 25-OH Vitamin D. The ELISA analytical biochemical
technique of the E0915Ge kit is based on 25-OH Vitamin D antibody-25-OH Vitamin D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 25-OH Vitamin D
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 25-OH Vitamin D. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH Vitamin D and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
105-116% |
104-115% |
103-113% |
100-109% |
|
EDTA plasma(n=5) |
92-100% |
110-120% |
108-117% |
105-114% |
|
heparin plasma(n=5) |
103-113%
|
96-106% |
104-104% |
93-105% |
General Annotation
Sub Unit:
N/A
Function:
Low blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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