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25OHVD (Shorten Name),
Product Name:

General 25OHVD/ 25-OH Vitamin D ELISA Kit

 
Cat.#:

E0915Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Calcifediol,Calcidiol

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200nmol/L
Sensitivity:
1.27nmol/L
Specificity:
Natural and recombinant general 25-OH Vitamin D
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0915Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (25-OH Vitamin D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 25-OH Vitamin D. The ELISA analytical biochemical technique of the E0915Ge kit is based on 25-OH Vitamin D antibody-25-OH Vitamin D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 25-OH Vitamin D antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 25-OH Vitamin D. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 25OHVD ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH Vitamin D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-116%

104-115%

103-113%

100-109%

EDTA plasma(n=5)

92-100%

110-120%

108-117%

105-114%

heparin plasma(n=5)

103-113%

 

96-106%

104-104%

93-105%

 

General Annotation


Sub Unit:
N/A


Function:
Low blood calcidiol (25-hydroxy-vitamin D) can result from avoiding the sun. Deficiency results in impaired bone mineralization, and leads to bone softening diseases.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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Sample Data
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