General DA/ Dopamine ELISA Kit

Cat.#:

E0851Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

7.8-500pg/mL

Sensitivity:

4.1pg/mL

Specificity:

Natural and recombinant general Dopamine

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0851Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Dopamine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dopamine. The ELISA analytical biochemical technique of the E0851Ge kit is based on Dopamine antibody-Dopamine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dopamine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Dopamine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General DA ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 2 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dopamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	117-127%	89-98%	106-114%	112-122%
EDTA plasma(n=5)	95-106%	94-103%	104-114%	94-104%
heparin plasma(n=5)	82-94%	111-120%	102-113%	91-101%

General Annotation

Sub Unit:

N/A

Function:

In the brain, dopamine functions as a neurotransmitter — a chemical released by nerve cells to send signals to other nerve cells. Dopamine is available as an intravenous medication acting on the sympathetic nervous system, producing effects such as increased heart rate and blood pressure.

Location:

Dopamine — a simple organic chemical in the catecholamine family — plays a number of important physiological roles in the bodies of animals. Its name derives from its chemical structure, which consists of an amine group (NH2) linked to a catechol structure called dihydroxyphenylalanine (acronym DOPA).

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