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IMA (Shorten Name),
Product Name:

Human IMA/ Ischemia Modified Albumin ELISA Kit

 
Cat.#:

E0825h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.1ng/mL
Specificity:
Natural and recombinant human Ischemia Modified Albumin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0825h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Ischemia Modified Albumin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ischemia Modified Albumin. The ELISA analytical biochemical technique of the E0825h kit is based on Ischemia Modified Albumin antibody-Ischemia Modified Albumin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ischemia Modified Albumin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Ischemia Modified Albumin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human IMA ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Ischemia Modified Albumin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ischemia Modified Albumin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

91-100%

89-98%

89-98%

96-106%

EDTA plasma(n=5)

111-120%

98-108%

100-109%

91-101%

heparin plasma(n=5)

103-113%

 

88-98%

112-112%

97-109%

 

General Annotation


Sub Unit:
N/A


Function:
IMA can be detected via the albumin cobalt binding (ACB) test, a limited available FDA approved assay. Myocardial ischemia alters the N-terminus of albumin reducing the ability of cobalt to bind to albumin. IMA measures ischemia in the blood vessels and thus returns results in minutes rather than traditional markers of necrosis that take hours. ACB test has low specificity therefore generating high number of false positives and must be used in conjunction with typical acute approaches such as ECG and physical exam. Additional studies are required.


Location:
N/A


Sample Data
Sample Data
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