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IMA (Shorten Name ),
Product Name:

Human IMA/ Ischemia Modified Albumin ELISA Kit

Cat.#:

E0825h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.1ng/mL
Specificity:
Natural and recombinant human Ischemia Modified Albumin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human IMA ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of Ischemia Modified Albumin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ischemia Modified Albumin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

91-100%

89-98%

89-98%

96-106%

EDTA plasma(n=5)

111-120%

98-108%

100-109%

91-101%

heparin plasma(n=5)

103-113%

 

88-98%

112-112%

97-109%

 

General Annotation


Sub Unit:
N/A


Function:
IMA can be detected via the albumin cobalt binding (ACB) test, a limited available FDA approved assay. Myocardial ischemia alters the N-terminus of albumin reducing the ability of cobalt to bind to albumin. IMA measures ischemia in the blood vessels and thus returns results in minutes rather than traditional markers of necrosis that take hours. ACB test has low specificity therefore generating high number of false positives and must be used in conjunction with typical acute approaches such as ECG and physical exam. Additional studies are required.


Location:
N/A


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