027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human GLP1 ELISA Kit
GLP1 (Shorten Name),
Product Name:

Human GLP1/ Glucagon-like peptide 1 ELISA Kit

 
Cat.#:

E0804h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.6-1000pg/mL
Sensitivity:
8.2pg/mL
Specificity:
Natural and recombinant human Glucagon-like peptide 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cardiovascular
Product Overview:
E0804h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Glucagon-like peptide 1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Glucagon-like peptide 1. The ELISA analytical biochemical technique of the E0804h kit is based on Glucagon-like peptide 1 antibody-Glucagon-like peptide 1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Glucagon-like peptide 1 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Glucagon-like peptide 1. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
Human GLP1 ELISA Kit
Package & Price: Sign in.
Attention Attention
×
Human GLP1 ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Glucagon-like peptide 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucagon-like peptide 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-120%

100-109%

86-97%

103-111%

EDTA plasma(n=5)

82-94%

86-96%

108-117%

86-96%

heparin plasma(n=5)

109-118%

 

96-105%

113-123%

106-114%

 

General Annotation


Sub Unit:
N/A


Function:
GLP-1 secretion by ileal L cells is dependent on the presence of nutrients in the lumen of the small intestine. The secretagogues (agents that cause or stimulate secretion) of this hormone include major nutrients like carbohydrate, protein and lipid. Once in the circulation, GLP-1 has a half-life of less than 2 minutes, due to rapid degradation by the enzyme dipeptidyl peptidase-4. It is a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. Such glucose-dependent action is particularly attractive because, when the plasma glucose concentration is in the normal fasting range, GLP-1 no longer stimulates insulin to cause hypoglycemia. GLP-1 appears to restore the glucose sensitivity of pancreatic β-cells, with the mechanism possibly involving the increased expression of GLUT2 and glucokinase. GLP-1 is also known to inhibit pancreatic β-cell apoptosis and stimulate the proliferation and differentiation of insulin-secreting β-cells. In addition, GLP-1 inhibits gastric secretion and motility. This delays and protracts carbohydrate absorption and contributes to a satiating effect.


Location:
Glucagon-like peptide-1 (GLP-1) is derived from the transcription product of the proglucagon gene. The major source of GLP-1 in the body is the intestinal L cell that secretes GLP-1 as a gut hormone. The biologically active forms of GLP-1 are: GLP-1-(7-37) and GLP-1-(7-36)NH2. Those peptides result from selective cleavage of the proglucagon molecule.


Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com