Hcy (Shorten Name),
Product Name:
General Hcy/ Homocysteine ELISA Kit
Cat.#:
E0772Ge
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/ml
Sensitivity:
0.38ng/ml
Specificity:
Natural and recombinant general Homocysteine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0772Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Homocysteine) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Homocysteine. The ELISA analytical biochemical
technique of the E0772Ge kit is based on Homocysteine antibody-Homocysteine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Homocysteine
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Homocysteine. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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- Data
- Citations ( 2 )
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.0%
Recovery
Recovery was determined by spiking various levels of Homocysteine into serum and plasma.
Recovery was determined by spiking various levels of Hcy into serum and plasma .
Sample Type | Average(%) | Recovery Range (%) |
Serum | 85 | 79-93 |
Plasma | 92 | 85-101 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Homocysteine and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 100-110% | 113-123% | 97-107% | 91-101% |
EDTA plasma(n=5) | 92-102% | 82-92% | 105-115% | 101-110% |
heparin plasma(n=5) | 107-117%
| 93-105% | 98-111% | 105-114% |
General Annotation
Sub Unit:
N/A
Function:
Homocysteine is a non-protein amino acid with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the amino acid cysteine, differing by an additional methylene (-CH2-) group. It is biosynthesized from methionine by the removal of its terminal Cε methyl group. Homocysteine can be recycled into methionine or converted into cysteine with the aid of B-vitamins. While detection of high levels of homocysteine has been linked to cardiovascular disease, lowering homocysteine levels may not improve outcomes.
Location:
N/A
Vaild Sequence:
[1].
Ying-Ying Xu, Dong-Yin Guan, Min Yang, Hao Wang, and Zong-Hou Shen
[2].
Ekim M, Ekim H, Yilmaz Y K, et al.
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