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Hcy (Shorten Name ),
Product Name:

General Hcy/ Homocysteine ELISA Kit

Cat.#:

E0772Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/ml
Sensitivity:
0.38ng/ml
Specificity:
Natural and recombinant general Homocysteine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General Hcy ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.0%


Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.0%

Recovery
Recovery was determined by spiking various levels of Homocysteine into serum and plasma.

Recovery was determined by spiking various levels of Hcy into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

85

79-93

Plasma

92

85-101

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Homocysteine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-110%

113-123%

97-107%

91-101%

EDTA plasma(n=5)

92-102%

82-92%

105-115%

101-110%

heparin plasma(n=5)

107-117%

 

93-105%

98-111%

105-114%

 

General Annotation


Sub Unit:
N/A


Function:
Homocysteine is a non-protein amino acid with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the amino acid cysteine, differing by an additional methylene (-CH2-) group. It is biosynthesized from methionine by the removal of its terminal Cε methyl group. Homocysteine can be recycled into methionine or converted into cysteine with the aid of B-vitamins. While detection of high levels of homocysteine has been linked to cardiovascular disease, lowering homocysteine levels may not improve outcomes.


Location:
N/A


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