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Hyl (Shorten Name),
Product Name:

General Hyl/ Hydroxylysine ELISA Kit

 
Cat.#:

E0742Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.053ng/mL
Specificity:
Natural and recombinant general Hydroxylysine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0742Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Hydroxylysine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Hydroxylysine. The ELISA analytical biochemical technique of the E0742Ge kit is based on Hydroxylysine antibody-Hydroxylysine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Hydroxylysine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Hydroxylysine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General Hyl ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hydroxylysine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

95-106%

107-118%

104-114%

93-102%

EDTA plasma(n=5)

107-118%

101-112%

96-107%

83-96%

heparin plasma(n=5)

92-101%

 

102-112%

86-96%

110-120%

 

General Annotation


Sub Unit:
N/A


Function:
Hydroxylysine is an amino acid with the molecular formula C6H14N2O3. It was first discovered in 1921 by Donald Van Slyke as the 5-Hydroxylysine form. It arises from a post-translational hydroxy modification of lysine. It is most widely known as a component of collagen.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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