General LTAD/ Lipoteichoic acid ELISA Kit

Cat.#:

E1405Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

LTA

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20ng/mL

Sensitivity:

0.079ng/mL

Specificity:

Natural and recombinant general Lipoteichoic acid

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E1405Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Lipoteichoic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Lipoteichoic acid. The ELISA analytical biochemical technique of the E1405Ge kit is based on Lipoteichoic acid antibody-Lipoteichoic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Lipoteichoic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Lipoteichoic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General LTAD ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lipoteichoic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-111%	101-113%	91-103%	87-95%
EDTA plasma(n=5)	103-115%	83-93%	94-103%	110-120%
heparin plasma(n=5)	105-114%	100-109%	92-101%	100-110%

General Annotation

Sub Unit:

N/A

Function:

LTA may bind to target cells non-specifically through membrane phospholipids, or specifically to CD14 and to Toll-like receptors. Binding to TLR-2 has shown to induce NF-κB expression(a central transcription factor), elevating expression of both pro- and anti-apoptotic genes. Its activation also induces mitogen-activated protein kinases (MAPK) activation along with Phosphoinositide 3-kinase activation.

Location:

Lipoteichoic acid (LTA) is a major constituent of the cell wall of Gram-positive bacteria. These organisms have an inner (or cytoplasmic) membrane and, external to it, a thick (up to 80 nanometer) peptidoglycan layer. The structure of LTA varies between the different species of Gram positive bacteria and may contain long chains of ribitol or glycerol phosphate. LTA is anchored to the cell membrane via a glyceride. It acts as regulator of autolytic wall enzymes (muramidases). It has antigenic properties being able to stimulate specific immune response and is released from the bacterial cells mainly after bacteriolysis induced by lysozyme, cationic peptides from leucocytes, or beta-lactam antibiotics.

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