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FK506 (Shorten Name),
Product Name:

General FK506/ Tacrolimus ELISA Kit

 
Cat.#:

E1207Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

fujimycin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/ml
Sensitivity:
0.069ng/ml
Specificity:
Natural and recombinant general Tacrolimus
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1207Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Tacrolimus) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tacrolimus. The ELISA analytical biochemical technique of the E1207Ge kit is based on Tacrolimus antibody-Tacrolimus antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tacrolimus antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Tacrolimus. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General FK506 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tacrolimus and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

85-95%

113-122%

107-116%

82-90%

EDTA plasma(n=5)

81-92%

82-92%

106-116%

101-112%

heparin plasma(n=5)

103-115%

 

81-90%

105-115%

95-103%

 

General Annotation


Sub Unit:
N/A


Function:
Tacrolimus (also FK-506 or fujimycin, trade names Prograf, Advagraf, Protopic) is an immunosuppressive drug that is mainly used after allogeneic organ transplant to reduce the activity of the patient's immune system and so lower the risk of organ rejection. It is also used in a topical preparation in the treatment of atopic dermatitis (eczema), severe refractory uveitis after bone marrow transplants, exacerbations of minimal change disease, and the skin condition vitiligo.


Location:
It is a 23-membered macrolide lactone discovered in 1984 from the fermentation broth of a Japanese soil sample that contained the bacteria Streptomyces tsukubaensis. It reduces interleukin-2 (IL-2) production by T-cells.


Sample Data
Sample Data
Sample Data
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