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PYD (Shorten Name),
Product Name:

General PYD/ Pyridinoline ELISA Kit

 
Cat.#:

E1037Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
6.25-400nmol/L
Sensitivity:
3.125nmol/L
Specificity:
Natural and recombinant general Pyridinoline
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1037Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Pyridinoline) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pyridinoline. The ELISA analytical biochemical technique of the E1037Ge kit is based on Pyridinoline antibody-Pyridinoline antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pyridinoline antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Pyridinoline. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General PYD ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pyridinoline and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

108-116%

95-106%

95-105%

EDTA plasma(n=5)

93-103%

108-118%

99-108%

90-100%

heparin plasma(n=5)

101-110%

 

92-104%

109-119%

96-96%

 

General Annotation


Sub Unit:
N/A


Function:
Pyridinoline and deoxypyridinoline were found to be released into the blood during bone degradation and rapidly exereted in the urine. In a preliminary study, both these compounds were proposed as a marker for metastatic bone tumor in patients with prostate cancer.


Location:
Pyridinoline is a fluorescent cross-linking compound of collagen fibers. Crosslinks in collagen and elastin are derived from lysyl and hydroxylysyl residues, a process catalyzed by lysyl oxidase. Fujimoto and colleagues first described the isolation and characterization of a fluorescent material in bovine achilles tendon collagen and termed it pyridinoline. It is reported to be present in collagen of bone and cartilage, but is absent in collagen of skin. It is not present in newly synthesized collagen and is formed from aldimine cross-links during maturation of collagen fibers.


Sample Data
Sample Data
Sample Data
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