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BHA (Shorten Name ),
Product Name:

General BHA/ beta-Hydroxybutyric acid ELISA Kit

Cat.#:

E1022Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

beta-hydroxybutyrate, 3-hydroxybutyric acid

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10umol/L
Sensitivity:
0.048umol/L
Specificity:
Natural and recombinant general beta-Hydroxybutyric acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General BHA ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-Hydroxybutyric acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-111%

114-126%

82-92%

92-101%

EDTA plasma(n=5)

99-110%

102-112%

102-112%

90-100%

heparin plasma(n=5)

94-103%

 

99-108%

101-111%

103-113%

 

General Annotation


Sub Unit:
N/A


Function:
In humans, beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA in a reaction catalyzed by the enzyme beta-hydroxybutyrate dehydrogenase and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis.


Location:
beta-Hydroxybutyric acid (also known as beta-hydroxybutyrate or 3-hydroxybutyric acid) is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-3-hydroxybutyric acid. Like the other ketone bodies (acetoacetate and acetone), levels of beta-hydroxybutyrate are raised in ketosis.


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