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BHA (Shorten Name),
Product Name:

General BHA/ beta-Hydroxybutyric acid ELISA Kit

 
Cat.#:

E1022Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

beta-hydroxybutyrate,3-hydroxybutyric acid

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10umol/L
Sensitivity:
0.048umol/L
Specificity:
Natural and recombinant general beta-Hydroxybutyric acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1022Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (beta-Hydroxybutyric acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing beta-Hydroxybutyric acid. The ELISA analytical biochemical technique of the E1022Ge kit is based on beta-Hydroxybutyric acid antibody-beta-Hydroxybutyric acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect beta-Hydroxybutyric acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, beta-Hydroxybutyric acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General BHA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta-Hydroxybutyric acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-111%

114-126%

82-92%

92-101%

EDTA plasma(n=5)

99-110%

102-112%

102-112%

90-100%

heparin plasma(n=5)

94-103%

 

99-108%

101-111%

103-113%

 

General Annotation


Sub Unit:
N/A


Function:
In humans, beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA in a reaction catalyzed by the enzyme beta-hydroxybutyrate dehydrogenase and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis.


Location:
beta-Hydroxybutyric acid (also known as beta-hydroxybutyrate or 3-hydroxybutyric acid) is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-3-hydroxybutyric acid. Like the other ketone bodies (acetoacetate and acetone), levels of beta-hydroxybutyrate are raised in ketosis.


Sample Data
Sample Data
Sample Data
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