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5HIAA (Shorten Name),
Product Name:

General 5HIAA/ 5-Hydroxyindoleacetic acid ELISA Kit

 
Cat.#:

E1005Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.625-40ng/mL
Sensitivity:
0.266ng/mL
Specificity:
Natural and recombinant general 5-Hydroxyindoleacetic acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1005Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (5-Hydroxyindoleacetic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-Hydroxyindoleacetic acid. The ELISA analytical biochemical technique of the E1005Ge kit is based on 5-Hydroxyindoleacetic acid antibody-5-Hydroxyindoleacetic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-Hydroxyindoleacetic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 5-Hydroxyindoleacetic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 5HIAA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 6.9

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 10.4

Recovery
Recovery was determined by spiking various levels of 5-Hydroxyindoleacetic acid into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-Hydroxyindoleacetic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

80-91%

103-113%

114-114%

100-110%

EDTA plasma(n=5)

101-110%

93-103%

91-100%

109-119%

heparin plasma(n=5)

105-115%

 

109-119%

87-99%

107-117%

 

General Annotation


Sub Unit:
N/A


Function:
5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin in the human body. In chemical analysis of urine samples, 5-HIAA is used to determine the body's levels of serotonin.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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