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CTXIII (Shorten Name),
Product Name:

Human CTXIII/ C-terminal telopeptide of Collagen alpha-1(III) chain ELISA Kit

 
Cat.#:

E1666h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.076ng/mL
Specificity:
Natural and recombinant human C-terminal telopeptide of Collagen alpha-1(III) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E1666h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (C-terminal telopeptide of Collagen alpha-1(III) chain) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C-terminal telopeptide of Collagen alpha-1(III) chain. The ELISA analytical biochemical technique of the E1666h kit is based on C-terminal telopeptide of Collagen alpha-1(III) chain antibody-C-terminal telopeptide of Collagen alpha-1(III) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C-terminal telopeptide of Collagen alpha-1(III) chain antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, C-terminal telopeptide of Collagen alpha-1(III) chain. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human CTXIII ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of C-terminal telopeptide of Collagen alpha-1(III) chain into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

106

100-112

Plasma

108

102-114

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C-terminal telopeptide of Collagen alpha-1(III) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-110%

96-105%

91-103%

102-114%

EDTA plasma(n=5)

105-113%

92-103%

99-107%

100-110%

heparin plasma(n=5)

112-120%

 

110-119%

110-120%

109-119%

 

General Annotation


Sub Unit:
N/A


Function:
In bone physiology, the C-terminal telopeptide (or more formally, carboxy-terminal collagen crosslinks, and known by the acronym CTX) is a serum biomarker used to measure the rate of bone turnover. It can be useful in assisting clinicians to determine a patient's nonsurgical treatment response as well as evaluate a patient's risk of developing complications during healing following surgical intervention. The test used to detect the CTX marker is called the Serum CrossLaps, and it is more specific to bone resorption than any other test currently available.


Location:
N/A


This product has not yet been referenced specifically in any publications.
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