027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  General VB12 ELISA Kit
VB12 (Shorten Name),
Product Name:

General VB12/ Vitamin B12 ELISA Kit

 
Cat.#:

E0924Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

cobalamin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/ml
Sensitivity:
0.051ng/ml
Specificity:
Natural and recombinant general Vitamin B12
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0924Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Vitamin B12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Vitamin B12. The ELISA analytical biochemical technique of the E0924Ge kit is based on Vitamin B12 antibody-Vitamin B12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Vitamin B12 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Vitamin B12. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
General VB12 ELISA Kit
Package & Price: Sign in.
Attention Attention
×
General VB12 ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 7.6%

Recovery
Recovery was determined by spiking various levels of Vitamin B12 into serum and plasma.

Recovery was determined by spiking various levels of VB12 into serum and plasma .

Sample Type

Average(%)

Recovery Range(%)

Serum

99

95-103

Plasma

98

97-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

86-98%

104-117%

104-116%

113-122%

EDTA plasma(n=5)

100-111%

103-113%

109-119%

99-110%

heparin plasma(n=5)

102-111%

 

91-100%

93-105%

83-92%

 

General Annotation


Sub Unit:
N/A


Function:
It is normally involved in the metabolism of every cell of the human body, especially affecting DNA synthesis and regulation, but also fatty acid synthesis and energy production.


Location:
Vitamin B12, vitamin B12 or vitamin B-12, also called cobalamin, is a water-soluble vitamin with a key role in the normal functioning of the brain and nervous system, and for the formation of blood. It is one of the eight B vitamins.


Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com