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ALD (Shorten Name),
Product Name:

General ALD/ Aldosterone ELISA Kit

 
Cat.#:

E0911Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.39-25ng/mL
Sensitivity:
0.194ng/mL
Specificity:
Natural and recombinant general Aldosterone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0911Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Aldosterone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Aldosterone. The ELISA analytical biochemical technique of the E0911Ge kit is based on Aldosterone antibody-Aldosterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Aldosterone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Aldosterone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General ALD ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aldosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-112%

111-121%

88-99%

81-90%

EDTA plasma(n=5)

89-102%

107-117%

107-119%

105-115%

heparin plasma(n=5)

105-116%

 

101-112%

105-117%

116-126%

 

General Annotation


Sub Unit:
N/A


Function:
Aldosterone is a yellow steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland, and acts mainly on the distal tubules and collecting ducts of the nephron, the functioning unit of the kidney, to cause the conservation of sodium, secretion of potassium, increased water retention, and increased blood pressure. The overall effect of aldosterone is to increase reabsorption of ions and water in the kidney -- increasing blood volume and, therefore, increasing blood pressure.


Location:
Steroid receptors are intracellular. The aldosterone mineralcorticoid receptor (MR) complex binds on the DNA to specific hormone response element, which leads to gene specific transcription.


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