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ALD (Shorten Name),

Product Name:

General ALD/ Aldosterone ELISA Kit

Cat.#:

E0911Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.39-25ng/mL

Sensitivity:

0.194ng/mL

Specificity:

Natural and recombinant general Aldosterone

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0911Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Aldosterone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Aldosterone. The ELISA analytical biochemical technique of the E0911Ge kit is based on Aldosterone antibody-Aldosterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Aldosterone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Aldosterone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General ALD ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aldosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-112%	111-121%	88-99%	81-90%
EDTA plasma(n=5)	89-102%	107-117%	107-119%	105-115%
heparin plasma(n=5)	105-116%	101-112%	105-117%	116-126%

General Annotation

Sub Unit:

N/A

Function:

Aldosterone is a yellow steroid hormone (mineralocorticoid family) produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland, and acts mainly on the distal tubules and collecting ducts of the nephron, the functioning unit of the kidney, to cause the conservation of sodium, secretion of potassium, increased water retention, and increased blood pressure. The overall effect of aldosterone is to increase reabsorption of ions and water in the kidney -- increasing blood volume and, therefore, increasing blood pressure.

Location:

Steroid receptors are intracellular. The aldosterone mineralcorticoid receptor (MR) complex binds on the DNA to specific hormone response element, which leads to gene specific transcription.

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Sequence length:

N/A

Sequence:

C21H28O5

3D Structure:

Predicted Eptitope:

Vaild Sequence:

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some questions for the ELISA kit

We have purchased the Human Aldosterone ELISA (Cat # E0911h) and the Human Renin ELISA kits (Cat # E0889h) and have some questions. 1. In the Sample collection and storage section of the package inserts for Plasma it states “Store samples at -20°C or -80°C.” Then under the Note: 1. “Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (1 month) or -80°C (2 months) to avoid loss of bioactivity and contamination.” Can you please clarify which sample type the Note 1. refers to? Can plasma samples be stored at 2-8°C for 5 days and used in the Aldosterone and Renin ELISAs? 2. Can you please provide stability data for plasma used in your Aldosterone and Renin ELISA kits? 3. In the Aldosterone ELISA, it is a competitive assay. In the Calculation of results section, it states “Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density.” Since it is a competitive assay this causes all of the data to be negative. Please advise how the calculations are performed. Can you provide an example? Thanks,

Asked by customer
2014-01-22 22:49

Review (1)

Thank you for your enquiry! 1.All samples could be stored at 4°C for short time,otherwise they should be stored at -20°C or -80°C . 'Can plasma samples be stored at 2-8°C for 5 days and used in the Aldosterone and Renin ELISAs? ' That is OK. 2.We could offer you the COA for the items in attachment for reference. 3.You could refer this link for your result analysis http://www.eiaab.com/info/detail/378 Hope this is helpful for you.Please contact us as free if you need any further information.

Answered by
2014-01-23 01:10