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MT (Shorten Name ),
Product Name:

General MT/ Melatonin ELISA Kit

Cat.#:

E0908Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant general Melatonin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General MT ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 7.3%

Recovery
Recovery was determined by spiking various levels of Melatonin into serum and plasma.

Recovery was determined by spiking various levels of Melatonin into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

101

82-109

Plasma

97

94-106

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melatonin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-106%

100-109%

96-104%

95-107%

EDTA plasma(n=5)

91-101%

107-116%

96-108%

88-100%

heparin plasma(n=5)

94-107%

 

82-93%

100-112%

98-109%

 

General Annotation


Sub Unit:
N/A


Function:
Many biological effects of melatonin are produced through activation of melatonin receptors, while others are due to its role as a pervasive and powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA.


Location:
Melatonin, also known chemically as N-acetyl-5-methoxytryptamine, is a naturally occurring compound found in animals, plants, and microbes. In animals, circulating levels of the hormone melatonin vary in a daily cycle, thereby allowing the entrainment of the circadian rhythms of several biological functions.


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