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MT (Shorten Name),
Product Name:

General MT/ Melatonin ELISA Kit

 
Cat.#:

E0908Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant general Melatonin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0908Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Melatonin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Melatonin. The ELISA analytical biochemical technique of the E0908Ge kit is based on Melatonin antibody-Melatonin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Melatonin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Melatonin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General MT ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 4.1

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 7.3

Recovery
Recovery was determined by spiking various levels of Melatonin into serum and plasma.

Recovery was determined by spiking various levels of Melatonin into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

101

82-109

Plasma

97

94-106

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Melatonin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-106%

100-109%

96-104%

95-107%

EDTA plasma(n=5)

91-101%

107-116%

96-108%

88-100%

heparin plasma(n=5)

94-107%

 

82-93%

100-112%

98-109%

 

General Annotation


Sub Unit:
N/A


Function:
Many biological effects of melatonin are produced through activation of melatonin receptors, while others are due to its role as a pervasive and powerful antioxidant, with a particular role in the protection of nuclear and mitochondrial DNA.


Location:
Melatonin, also known chemically as N-acetyl-5-methoxytryptamine, is a naturally occurring compound found in animals, plants, and microbes. In animals, circulating levels of the hormone melatonin vary in a daily cycle, thereby allowing the entrainment of the circadian rhythms of several biological functions.


Sample Data
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