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HbA1c (Shorten Name),
Product Name:

Human HbA1c/ Glycosylated hemoglobin A1c ELISA Kit

 
Cat.#:

E0897h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.9ng/mL
Specificity:
Natural and recombinant human Glycosylated hemoglobin A1c
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0897h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Glycosylated hemoglobin A1c) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Glycosylated hemoglobin A1c. The ELISA analytical biochemical technique of the E0897h kit is based on Glycosylated hemoglobin A1c antibody-Glycosylated hemoglobin A1c antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Glycosylated hemoglobin A1c antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Glycosylated hemoglobin A1c. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human HbA1c ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.7%

Recovery
Recovery was determined by spiking various levels of Glycosylated hemoglobin A1c into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glycosylated hemoglobin A1c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-118%

108-119%

110-119%

89-99%

EDTA plasma(n=5)

86-96%

88-88%

101-109%

109-119%

heparin plasma(n=5)

90-98%

 

96-106%

104-112%

104-116%

 

General Annotation


Sub Unit:
N/A


Function:
In the normal 120-day lifespan of the red blood cell, glucose molecules react with hemoglobin, forming glycosolated hemoglobin. In individuals with poorly controlled diabetes, the quantities of these glycosolated hemoglobins are much higher than in healthy people.


Location:
Glycosylated hemoglobin (hemoglobin A1c, HbA1c, A1C, or Hb1c; sometimes also HbA1c) is a form of hemoglobin that is measured primarily to identify the average plasma glucose concentration over prolonged periods of time. It is formed in a non-enzymatic glycation pathway by hemoglobin's exposure to plasma glucose. Normal levels of glucose produce a normal amount of glycosolated hemoglobin. As the average amount of plasma glucose increases, the fraction of glycosolated hemoglobin increases in a predictable way. This serves as a marker for average blood glucose levels over the previous months prior to the measurement.


Sample Data
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