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PGI2 (Shorten Name),
Product Name:

General PGI2/ Prostacyclin ELISA Kit

 
Cat.#:

E0727Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
31.2-2000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant general Prostacyclin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0727Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Prostacyclin) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prostacyclin. The ELISA analytical biochemical technique of the E0727Ge kit is based on Prostacyclin antibody-Prostacyclin antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prostacyclin antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Prostacyclin. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General PGI2 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostacyclin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-108%

106-118%

100-108%

89-98%

EDTA plasma(n=5)

102-114%

108-118%

94-106%

88-97%

heparin plasma(n=5)

112-122%

 

100-110%

90-100%

83-93%

 

General Annotation


Sub Unit:
N/A


Function:
Prostacyclin (PGI2) chiefly prevents formation of the platelet plug involved in primary hemostasis (a part of blood clot formation). It does this by inhibiting platelet activation. It is also an effective vasodilator. Prostacyclin's interactions in contrast to thromboxane (TXA2), another eicosanoid, strongly suggest a mechanism of cardiovascular homeostasis between the two hormones in relation to vascular damage.


Location:
Prostacyclin is produced in endothelial cells from prostaglandin H2 (PGH2) by the action of the enzyme prostacyclin synthase. Although prostacyclin is considered an independent mediator, it is called PGI2 (prostaglandin I2) in eicosanoid nomenclature, and is a member of the prostanoids (together with the prostaglandins and thromboxane).


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