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CS (Shorten Name),
Product Name:

General CS/ Chondroitin sulfate ELISA Kit

 
Cat.#:

E0723Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.19ng/mL
Specificity:
Natural and recombinant general Chondroitin sulfate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0723Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Chondroitin sulfate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Chondroitin sulfate. The ELISA analytical biochemical technique of the E0723Ge kit is based on Chondroitin sulfate antibody-Chondroitin sulfate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Chondroitin sulfate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Chondroitin sulfate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General CS ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of Chondroitin sulfate into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

87

81-93

Plasma

89

83-95

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chondroitin sulfate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

105-114%

105-114%

100-110%

107-107%

EDTA plasma(n=5)

104-114%

93-102%

95-106%

93-102%

heparin plasma(n=5)

110-119%

 

97-108%

86-95%

107-116%

 

General Annotation


Sub Unit:
N/A


Function:
Chondroitin sulfate readily interacts with proteins in the extracellular matrix due to its negative charges. These interactions are important for regulating a diverse array of cellular activities. The lecticans are a major part of the brain extracellular matrix, where the chondroitin sugar chains function to stabilize normal brain synapses as part of perineuronal nets. The levels of chondroitin sulfate proteoglycans are vastly increased after injury to the central nervous system where they act to prevent regeneration of damaged nerve endings. Although these functions are not as well characterized as those of heparan sulfate, new roles continue to be discovered for the chondroitin sulfate proteoglycans.


Location:
Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars (N-acetylgalactosamine and glucuronic acid). It is usually found attached to proteins as part of a proteoglycan. A chondroitin chain can have over 100 individual sugars, each of which can be sulfated in variable positions and quantities. Chondroitin sulfate is an important structural component of cartilage and provides much of its resistance to compression. Along with glucosamine, chondroitin sulfate has become a widely used dietary supplement for treatment of osteoarthritis.


This product has not yet been referenced specifically in any publications.
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[1].
"Single nucleotide polymorphisms in succinate dehydrogenase subunits and citrate synthase genes: association results for impaired spermatogenesis."

[2].
"[Cloning and tissue expression pattern analysis of the human citrate synthase cDNA]."

[3].
"Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase."

[4].
"Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation."

[5].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[6].
"Identification and characterization of the mitochondrial targeting sequence and mechanism in human citrate synthase."

[7].
"Interactive domains for chaperone activity in the small heat shock protein, human alphaB crystallin."

[8].
"Enhanced citrate synthase activity in human pancreatic cancer."

[9].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[10].
"Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes."
Sample Data
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