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AMSH (Shorten Name),
Product Name:

Human AMSH/ alpha-MSH ELISA Kit

 
Cat.#:

E0239h

Brand:
EIAab®
Regulatory Status:
Alternative:

alpha-melanotropin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
1.56-100ng/mL
Sensitivity:
0.6ng/mL
Specificity:
Natural and recombinant human alpha-MSH
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0239h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (alpha-MSH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing alpha-MSH. The ELISA analytical biochemical technique of the E0239h kit is based on alpha-MSH antibody-alpha-MSH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect alpha-MSH antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, alpha-MSH. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human AMSH ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Recovery
Recovery was determined by spiking various levels of alpha-MSH into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of alpha-MSH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-99%

106-116%

108-117%

85-93%

EDTA plasma(n=5)

88-98%

104-116%

89-99%

105-117%

heparin plasma(n=5)

106-116%

 

97-107%

107-107%

92-102%

 

General Annotation


Sub Unit:
N/A


Function:
It is the most important of the melanocyte-stimulating hormones (MSHs; also known as melanotropins) in stimulating melanogenesis, a process which in mammals (including humans) is responsible for pigmentation primarily of the hair and skin. It also plays a role in feeding behavior, energy homeostasis, and sexual activity.


Location:
It is generated as a proteolyic cleavage product from ACTH (1-13), which is in turn a cleavage product of proopiomelanocortin (POMC).


This product has not yet been referenced specifically in any publications.
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