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首页  >  酶联免疫试剂盒  >  General HYP ELISA Kit
HYP (短名 ),
产品名称:

General HYP/ Hydroxyproline ELISA Kit
羟脯氨酸

货号:

E0621Ge

商标:
EIAab®
监管等级:
检测方法:
ELISA
实验类型:
Competitive
检测范围:
62.5-4000nmol/L
灵敏度:
31.25nmol/L
特异性:
Natural and recombinant general Hydroxyproline
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
-
General HYP ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

111-120%

102-112%

85-94%

100-111%

EDTA plasma(n=5)

109-118%

92-104%

113-123%

106-115%

heparin plasma(n=5)

93-105%

 

101-111%

99-110%

96-108%

 

通用注释


亚单元:
N/A


功能:
Hydroxyproline is a major component of the protein collagen. Hydroxyproline and proline play key roles for collagen stability. They permit the sharp twisting of the collagen helix. Hydroxyproline is found in few proteins other than collagen. For this reason, hydroxyproline content has been used as an indicator to determine collagen and/or gelatin amount.


Location:
Hydroxyproline is produced by hydroxylation of the amino acid proline by the enzyme prolyl hydroxylase following protein synthesis (as a post-translational modification). The enzyme catalysed reaction takes place in the lumen of the endoplasmic reticulum. Although it is not directly incorporated into proteins, hydroxyproline comprises roughly 4% of all amino acids found in animal tissue, more than seven amino acids which are directly incorporated.


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