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HIS (Shorten Name),
Product Name:

General HIS/ Histamine ELISA Kit

 
Cat.#:

E0927Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
7.8-500ng/mL
Sensitivity:
3.4ng/mL
Specificity:
Natural and recombinant general Histamine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0927Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Histamine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Histamine. The ELISA analytical biochemical technique of the E0927Ge kit is based on Histamine antibody-Histamine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Histamine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Histamine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General HIS ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 11%

Recovery
Recovery was determined by spiking various levels of Histamine into serum and plasma.

Recovery was determined by spiking various levels of Histamine into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

92

89-101

Plasma

87

82-96

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Histamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-97%

87-96%

103-112%

96-105%

EDTA plasma(n=5)

80-91%

96-106%

102-111%

87-99%

heparin plasma(n=5)

95-104%

 

97-107%

111-120%

106-118%

 

General Annotation


Sub Unit:
N/A


Function:
Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response.


Location:
As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.


Sample Data
Sample Data
Sample Data
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