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HIS (Shorten Name ),
Product Name:

General HIS/ Histamine ELISA Kit

Cat.#:

E0927Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
7.8-500ng/mL
Sensitivity:
3.4ng/mL
Specificity:
Natural and recombinant general Histamine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
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General HIS ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 11%

Recovery
Recovery was determined by spiking various levels of Histamine into serum and plasma.

Recovery was determined by spiking various levels of Histamine into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

92

89-101

Plasma

87

82-96

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Histamine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-97%

87-96%

103-112%

96-105%

EDTA plasma(n=5)

80-91%

96-106%

102-111%

87-99%

heparin plasma(n=5)

95-104%

 

97-107%

111-120%

106-118%

 

General Annotation


Sub Unit:
N/A


Function:
Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response.


Location:
As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.


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