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8OHDG (Shorten Name),
Product Name:

General 8OHDG/ 8-Oxo-2'-deoxyguanosine ELISA Kit

 
Cat.#:

E0660Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

8-Hydroxydeoxyguanosine

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.27ng/mL
Specificity:
Natural and recombinant general 8-Oxo-2'-deoxyguanosine
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0660Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (8-Oxo-2'-deoxyguanosine) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 8-Oxo-2'-deoxyguanosine. The ELISA analytical biochemical technique of the E0660Ge kit is based on 8-Oxo-2'-deoxyguanosine antibody-8-Oxo-2'-deoxyguanosine antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 8-Oxo-2'-deoxyguanosine antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 8-Oxo-2'-deoxyguanosine. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General 8OHDG ELISA Kit
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Product Datasheets
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-Oxo-2'-deoxyguanosine and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-111%

101-110%

105-115%

107-117%

EDTA plasma(n=5)

102-112%

91-102%

94-104%

102-112%

heparin plasma(n=5)

105-115%

 

104-115%

85-95%

99-108%

 

General Annotation


Sub Unit:
N/A


Function:
8-Oxo-2'-deoxyguanosine (8-oxo-dG) is an oxidized derivative of deoxyguanosine. 8-oxo-dG is one of the major products of DNA oxidation. Concentrations of 8-oxo-dG within a cell are a measurement of oxidative stress.


Location:
N/A



[3].
Su-Ping Zhang, Yan-WenWu, Zhao-ZhaoWu, Hai-Yun Liu, Ji-Hua Nie, Jian Tong
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