首页 > 酶联免疫试剂盒 > General HS ELISA Kit

HS (短名 ),

产品名称:

General HS/ Heparan sulfate ELISA Kit
硫酸类肝素

货号:

E0623Ge

商标:

EIAab^®

监管等级:

RUO

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

4.69-300ng/mL

灵敏度:

2.345ng/mL

特异性:

Natural and recombinant general Heparan sulfate

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据表:

说明书:

MSDS:

数据
文献 ( 1 )
序列 / 3D
常见问题解答
反馈墙 (4)

精密度

批内差：已知浓度的3个样本在一个板子内重复检测20次，以评估批内精密度。
批内 CV： ≤3.8%
批间差：已知浓度的3个样本在不同的板子上重复测定5次，以评估测定批间精密度。
批间 CV： ≤7.6%

回收率

回收率：低、中和高浓度的分析物被掺入到血清或者血浆样本中，进行回收实验测定。

Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	85	79-93
Plasma	92	85-101

线性

线性：给定样本通过梯度稀释，每次稀释的测量值与理论值的比值。

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-102%	91-100%	95-105%	100-110%
EDTA plasma(n=5)	93-105%	102-114%	107-117%	104-113%
heparin plasma(n=5)	91-101%	100-113%	111-120%	101-110%

通用注释

亚单元:

N/A

功能:

Heparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (HSPG) in which two or three HS chains are attached in close proximity to cell surface or extracellular matrix proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation and tumour metastasis. HS has been shown to serve as cellular receptor for a number of viruses including the respiratory syncytial virus.

Location:

N/A

相关产品

General HS CLIA Kit

Human C19orf80 ELISA Kit

Human ASPRO ELISA Kit

Mouse Trap1a ELISA Kit

Human ACE ELISA Kit

序列长度:

N/A

序列:

N/A

3D 模型:

3D 结构:

免疫原序列:

验证序列:

[1].

"Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly"

PR Amable, MV Teixeira, RB Carias, et al.

From: Stem cell research & therapy

[1].

通用硫酸类肝素(HS)ELISA试剂盒可以做多少个样本？

通用硫酸类肝素(HS)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

通用硫酸类肝素(HS)ELISA试剂盒使用视频？

通用硫酸类肝素(HS)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

通用硫酸类肝素(HS)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的通用硫酸类肝素(HS)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

通用硫酸类肝素(HS)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

通用硫酸类肝素(HS)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析通用硫酸类肝素(HS)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

通用硫酸类肝素(HS)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集通用硫酸类肝素(HS)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

通用硫酸类肝素(HS)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

通用硫酸类肝素(HS)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

通用硫酸类肝素(HS)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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评论数 : 4

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customer

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最近

早期

old E0623h and new E0623Ge

Many thanks for your email and the attached paper. Checking the reference I note that it refers to an old catalogue number (saying: “Extracellular matrix proteins were also quantified in supernatants using commercial ELISA kits and following the manufacturers’ instructions: heparan sulphate (E0623h,EIAab, Wuhan, Hubei, China)…”) rather than with the current catalogue number E0623Ge… Can you confirm whether old E0623h and new E0623Ge are the same kit under different catalogue numbers?

customer
2015-03-10 19:24

Dear customer,Thank you for your feedback. Heparan sulfate is essential for all living organisms and it is not a species specific protein.We have updated the product website and the product for human Heparan sulfate is Cat.:E0623Ge. It should work with human samples,too.Please contact us as free if you need any further information.

star
2015-03-12 00:28

Technical enquiry about the ELISA Kit for General Heparan sulfate

My customer has a technical enquiry about the ELISA Kit for General Heparan sulfate (Cat. E0623GE). On your protocol at the top of page number 3, you specified in bold text the following warning : “The reconstituted Standards Detection Reagent A and B can be used only once.” In regards to the above statement, my customer has the following two questions : 1.- Does this mean that the reconstituted standards, AND the Detection Reagent A (diluted 1:100) AND the Detection Reagent B (diluted 1:100) can only be used once? 2.- Is it possible to reconstitute all 3 reagents and store 4 degrees Celcius for a day or two if some remains for another attempt at assay? Thank you for your assistance and looking forward to hearing from you!

customer
2015-01-04 00:24

1.The reconstituted standards, AND the Detection Reagent A (diluted 1:100) AND the Detection Reagent B (diluted 1:100) can only be used once. 2. I would recommend diluting the required amount based on the volume used for the assay.

star
2015-01-04 22:41

the concentration and the composition of the stop solution

My customer would like to know both the concentration and the composition of the stop solution from the ELISA Kit for General Heparan sulfate (Cat. E0623GE). Can you please provide me with both information?

customer
2014-06-15 19:48

Dear customer, Thank you for your inquiry. The stop solution for the ELISA kit is 2mol/L H2SO4. Hope this information is useful for you.

star
2014-12-21 19:17

some concerns for this kit

I have read the information provided with the kit and this raises some concerns. Please explain how you can have recombinant protein as the reference standard when the assay is for heparan sulfate. Is the assay really for HSPG? Which HSPG? If so it is not suitable for our requirements.Please provide information on cross-reactivity with heparan sulfate from other species or from differing organs.

customer
2014-01-20 00:50

The Human heparan sulfate ELISA is designed against the in-vitro quantitative determination of heparin sulfatein human serum, plasma, buffered solutions or cell culture medium. The assay is highly specific for Heparan Sulfate Proteoglycan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin. Ihope this information has been of help. If you require any further information please do not hesitate to contact us again

2014-01-20 22:28