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HS (Shorten Name ),
Product Name:

General HS/ Heparan sulfate ELISA Kit

Cat.#:

E0623Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
4.69-300ng/mL
Sensitivity:
2.345ng/mL
Specificity:
Natural and recombinant general Heparan sulfate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General HS ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.8%


Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Recovery
Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma.

Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

85

79-93

Plasma

92

85-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparan sulfate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-102%

91-100%

95-105%

100-110%

EDTA plasma(n=5)

93-105%

102-114%

107-117%

104-113%

heparin plasma(n=5)

91-101%

 

100-113%

111-120%

101-110%

 

General Annotation


Sub Unit:
N/A


Function:
Heparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (HSPG) in which two or three HS chains are attached in close proximity to cell surface or extracellular matrix proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation and tumour metastasis. HS has been shown to serve as cellular receptor for a number of viruses including the respiratory syncytial virus.


Location:
N/A


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