HS (Shorten Name),
Product Name:
General HS/ Heparan sulfate ELISA Kit
Cat.#:
E0623Ge
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
4.69-300ng/mL
Sensitivity:
2.345ng/mL
Specificity:
Natural and recombinant general Heparan sulfate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0623Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Heparan sulfate) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Heparan sulfate. The ELISA analytical biochemical
technique of the E0623Ge kit is based on Heparan sulfate antibody-Heparan sulfate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Heparan sulfate
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Heparan sulfate. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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- Data
- Citations ( 1 )
- Sequence / 3D
- Feedback Wall (4)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.6%
Recovery
Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma.
Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma .
Sample Type | Average(%) | Recovery Range (%) |
Serum | 85 | 79-93 |
Plasma | 92 | 85-101 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparan sulfate and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1：2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 90-102% | 91-100% | 95-105% | 100-110% |
EDTA plasma(n=5) | 93-105% | 102-114% | 107-117% | 104-113% |
heparin plasma(n=5) | 91-101%
| 100-113% | 111-120% | 101-110% |
General Annotation
Sub Unit:
N/A
Function:
Heparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (HSPG) in which two or three HS chains are attached in close proximity to cell surface or extracellular matrix proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation and tumour metastasis. HS has been shown to serve as cellular receptor for a number of viruses including the respiratory syncytial virus.
Location:
N/A
Vaild Sequence:
[1].
PR Amable, MV Teixeira, RB Carias, et al.
Feedback Wall
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old E0623h and new E0623Ge
Many thanks for your email and the attached paper. Checking the reference I note that it refers to an old catalogue number (saying: “Extracellular matrix proteins were also quantified in supernatants using commercial ELISA kits and following the manufacturers’ instructions: heparan sulphate (E0623h,EIAab, Wuhan, Hubei, China)…”) rather than with the current catalogue number E0623Ge…
Can you confirm whether old E0623h and new E0623Ge are the same kit under different catalogue numbers?
Asked by customer
2015-03-10 19:24
2015-03-10 19:24
Dear customer,Thank you for your feedback. Heparan sulfate is essential for all living organisms and it is not a species specific protein.We have updated the product website and the product for human Heparan sulfate is Cat.:E0623Ge. It should work with human samples,too.Please contact us as free if you need any further information.
Answered by star
2015-03-12 00:28
2015-03-12 00:28
0
0
Technical enquiry about the ELISA Kit for General Heparan sulfate
My customer has a technical enquiry about the ELISA Kit for General Heparan sulfate (Cat. E0623GE). On your protocol at the top of page number 3, you specified in bold text the following warning :
“The reconstituted Standards Detection Reagent A and B can be used only once.”
In regards to the above statement, my customer has the following two questions :
1.- Does this mean that the reconstituted standards, AND the Detection Reagent A (diluted 1:100) AND the Detection Reagent B (diluted 1:100) can only be used once?
2.- Is it possible to reconstitute all 3 reagents and store 4 degrees Celcius for a day or two if some remains for another attempt at assay?
Thank you for your assistance and looking forward to hearing from you!
Asked by customer
2015-01-04 00:24
2015-01-04 00:24
1.The reconstituted standards, AND the Detection Reagent A (diluted 1:100) AND the Detection Reagent B (diluted 1:100) can only be used once.
2. I would recommend diluting the required amount based on the volume used for the assay.
Answered by star
2015-01-04 22:41
2015-01-04 22:41
0
0
the concentration and the composition of the stop solution
My customer would like to know both the concentration and the composition of the stop solution from the ELISA Kit for General Heparan sulfate (Cat. E0623GE).
Can you please provide me with both information?
Asked by customer
2014-06-15 19:48
2014-06-15 19:48
Dear customer,
Thank you for your inquiry.
The stop solution for the ELISA kit is 2mol/L H2SO4.
Hope this information is useful for you.
Answered by star
2014-12-21 19:17
2014-12-21 19:17
0
0
some concerns for this kit
I have read the information provided with the kit and this raises some concerns. Please explain how you can have recombinant protein as the reference standard when the assay is for heparan sulfate. Is the assay really for HSPG? Which HSPG? If so it is not suitable for our requirements.Please provide information on cross-reactivity with heparan sulfate from other species or from differing organs.
Asked by customer
2014-01-20 00:50
2014-01-20 00:50
The Human heparan sulfate ELISA is designed against the in-vitro quantitative determination of heparin sulfatein human serum, plasma, buffered solutions or cell culture medium. The assay is highly specific for Heparan Sulfate Proteoglycan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin.
Ihope this information has been of help. If you require any further information please do not hesitate to contact us again
Answered by
2014-01-20 22:28
2014-01-20 22:28
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