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HS (Shorten Name),
Product Name:

General HS/ Heparan sulfate ELISA Kit

 
Cat.#:

E0623Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
4.69-300ng/mL
Sensitivity:
2.345ng/mL
Specificity:
Natural and recombinant general Heparan sulfate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0623Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Heparan sulfate) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Heparan sulfate. The ELISA analytical biochemical technique of the E0623Ge kit is based on Heparan sulfate antibody-Heparan sulfate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Heparan sulfate antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Heparan sulfate. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General HS ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.8%


Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.6%

Recovery
Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma.

Recovery was determined by spiking various levels of Heparan sulfate into serum and plasma .

Sample Type

Average(%)

Recovery  Range (%)

Serum

85

79-93

Plasma

92

85-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparan sulfate and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-102%

91-100%

95-105%

100-110%

EDTA plasma(n=5)

93-105%

102-114%

107-117%

104-113%

heparin plasma(n=5)

91-101%

 

100-113%

111-120%

101-110%

 

General Annotation


Sub Unit:
N/A


Function:
Heparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (HSPG) in which two or three HS chains are attached in close proximity to cell surface or extracellular matrix proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation and tumour metastasis. HS has been shown to serve as cellular receptor for a number of viruses including the respiratory syncytial virus.


Location:
N/A


Sample Data
Sample Data
Sample Data
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