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FA (Shorten Name),
Product Name:

General FA/ Folic acid ELISA Kit

 
Cat.#:

E0610Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

Vitamin B9,Vitamin Bc,Folacin

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.12ng/mL
Specificity:
Natural and recombinant general Folic acid
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0610Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Folic acid) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Folic acid. The ELISA analytical biochemical technique of the E0610Ge kit is based on Folic acid antibody-Folic acid antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Folic acid antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Folic acid. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General FA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Folic acid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

89-99%

96-105%

107-116%

97-105%

EDTA plasma(n=5)

106-116%

97-107%

107-117%

96-104%

heparin plasma(n=5)

116-126%

 

91-101%

98-109%

82-93%

 

General Annotation


Sub Unit:
N/A


Function:
Vitamin B9 (folic acid and folate inclusive) is essential to numerous bodily functions. The human body needs folate to synthesize DNA, repair DNA, and methylate DNA as well as to act as a cofactor in biological reactions involving folate. It is especially important in aiding rapid cell division and growth, such as in infancy and pregnancy. Children and adults both require folic acid to produce healthy red blood cells and prevent anemia.


Location:
Folic acid (also known as vitamin B9, vitamin Bc or folacin) and folate (the form naturally occurring in the body), as well as pteroyl-L-glutamic acid, pteroyl-L-glutamate, and pteroylmonoglutamic acid are forms of the water-soluble vitamin B9. Folic acid is itself not biologically active, but its biological importance is due to tetrahydrofolate and other derivatives after its conversion to dihydrofolic acid in the liver.


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