General MDA/ Malondialdehyde ELISA Kit

Cat.#:

E0597Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20nmol/mL

Sensitivity:

0.088nmol/mL

Specificity:

Natural and recombinant general Malondialdehyde

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0597Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Malondialdehyde) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Malondialdehyde. The ELISA analytical biochemical technique of the E0597Ge kit is based on Malondialdehyde antibody-Malondialdehyde antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Malondialdehyde antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Malondialdehyde. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General MDA ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations ( 9 )
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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Malondialdehyde and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	108-116%	95-107%	105-115%	94-104%
EDTA plasma(n=5)	106-118%	103-113%	81-93%	98-98%
heparin plasma(n=5)	99-111%	107-117%	101-111%	98-108%

General Annotation

Sub Unit:

N/A

Function:

Malondialdehyde reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, the primary one being M1G, which is mutagenic. The guanidine group of arginine residues condense with MDA to give 2-aminopyrimidines.

Location:

Malondialdehyde is the organic compound with the formula CH2(CHO)2. The structure of this species is more complex than this formula suggests. This reactive species occurs naturally and is a marker for oxidative stress.

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