Human PINP/ N-terminal propeptide of Collagen alpha-1(I) chain ELISA Kit

Cat.#:

E0957h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

3.12-200ng/mL

Sensitivity:

1.6ng/mL

Specificity:

Natural and recombinant human N-terminal propeptide of Collagen alpha-1(I) chain

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Human PINP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.5%

Recovery

Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(I) chain into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	106	100-112
Plasma	108	102-114

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(I) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-105%	88-100%	108-118%	111-120%
EDTA plasma(n=5)	108-108%	108-117%	104-114%	97-107%
heparin plasma(n=5)	101-111%	99-109%	106-116%	98-110%