Human PIIINP/ N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit

Cat.#:

E0573h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20ng/mL

Sensitivity:

0.21ng/mL

Specificity:

Natural and recombinant human N-terminal propeptide of Collagen alpha-1(III) chain

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0573h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N-terminal propeptide of Collagen alpha-1(III) chain. The ELISA analytical biochemical technique of the E0573h kit is based on N-terminal propeptide of Collagen alpha-1(III) chain antibody-N-terminal propeptide of Collagen alpha-1(III) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N-terminal propeptide of Collagen alpha-1(III) chain antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N-terminal propeptide of Collagen alpha-1(III) chain. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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Human PIIINP ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Recovery

Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(III) chain into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-104
Plasma	100	94-106

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(III) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	103-112%	104-114%	102-111%	106-115%
EDTA plasma(n=5)	89-101%	109-118%	113-123%	113-123%
heparin plasma(n=5)	103-111%	91-101%	107-117%	93-102%