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PIIINP (Shorten Name),
Product Name:

Human PIIINP/ N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit

 
Cat.#:

E0573h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.21ng/mL
Specificity:
Natural and recombinant human N-terminal propeptide of Collagen alpha-1(III) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0573h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing N-terminal propeptide of Collagen alpha-1(III) chain. The ELISA analytical biochemical technique of the E0573h kit is based on N-terminal propeptide of Collagen alpha-1(III) chain antibody-N-terminal propeptide of Collagen alpha-1(III) chain antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect N-terminal propeptide of Collagen alpha-1(III) chain antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, N-terminal propeptide of Collagen alpha-1(III) chain. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human PIIINP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.1%

Recovery
Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(III) chain into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

98

92-104

Plasma

100

94-106

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(III) chain and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

103-112%

104-114%

102-111%

106-115%

EDTA plasma(n=5)

89-101%

109-118%

113-123%

113-123%

heparin plasma(n=5)

103-111%

 

91-101%

107-117%

93-102%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Location:
N/A


Sample Data
Sample Data
Sample Data
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