PIIINP (Shorten Name ),
Product Name:
Human PIIINP/ N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit
Cat.#:
E0573h
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.21ng/mL
Specificity:
Natural and recombinant human N-terminal propeptide of Collagen alpha-1(III) chain
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations ( 2 )
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
Recovery
Recovery was determined by spiking various levels of N-terminal propeptide of Collagen alpha-1(III) chain into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
98 |
92-104 |
Plasma |
100 |
94-106 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-terminal propeptide of Collagen alpha-1(III) chain and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
103-112% |
104-114% |
102-111% |
106-115% |
EDTA plasma(n=5) |
89-101% |
109-118% |
113-123% |
113-123% |
heparin plasma(n=5) |
103-111%
|
91-101% |
107-117% |
93-102% |
General Annotation
Sub Unit:
N/A
Function:
N/A
Location:
N/A
[1].
K Taranta-Janusz, A Moczulska, H Nosek, et al.
[2].
Keyte S V, Kenny P J, Forcada Y, et al.
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