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IgA (Shorten Name),
Product Name:

Human IgA/ Immunoglobulin A ELISA Kit

 
Cat.#:

E0546h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
62.5-4000pg/mL
Specificity:
Natural and recombinant human Immunoglobulin A
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0546h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin A. The ELISA analytical biochemical technique of the E0546h kit is based on Immunoglobulin A antibody-Immunoglobulin A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin A antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin A. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human IgA ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin A into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

86-95%

103-113%

104-114%

107-116%

EDTA plasma(n=5)

98-106%

118-127%

107-115%

108-118%

heparin plasma(n=5)

98-107%

 

96-108%

108-108%

100-110%

 

General Annotation


Sub Unit:
N/A


Function:
Decreased or absent IgA, termed selective IgA deficiency, can be a clinically significant immunodeficiency.


Location:
Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. More IgA is produced in mucosal linings than all other types of antibody combined; between three and five grams are secreted into the intestinal lumen each day. This accumulates to 75% of the total immunoglobulin produced in the entire body.


Sample Data
Sample Data
Sample Data
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