Human IgE/ Immunoglobulin E ELISA Kit

Cat.#:

E0545h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.78ng/mL

Specificity:

Natural and recombinant human Immunoglobulin E

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Immunology

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Human IgE ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 1 )
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.5%

Recovery

Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	113	107-119
Plasma	115	109-120

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-112%	109-119%	86-96%	95-104%
EDTA plasma(n=5)	91-101%	108-118%	94-103%	86-99%
heparin plasma(n=5)	99-109%	101-113%	90-99%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.

Location:

Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).

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