027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human IgE ELISA Kit
IgE (Shorten Name),
Product Name:

Human IgE/ Immunoglobulin E ELISA Kit

 
Cat.#:

E0545h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.78ng/mL
Specificity:
Natural and recombinant human Immunoglobulin E
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0545h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin E) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin E. The ELISA analytical biochemical technique of the E0545h kit is based on Immunoglobulin E antibody-Immunoglobulin E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin E antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin E. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
More
Human IgE ELISA Kit
Package & Price: Sign in.
Attention Attention
×
Human IgE ELISA Kit
Email *
Message *
Please Sign in.
Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.5%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

113

107-119

Plasma

115

109-120

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-112%

109-119%

86-96%

95-104%

EDTA plasma(n=5)

91-101%

108-118%

94-103%

86-99%

heparin plasma(n=5)

99-109%

 

101-113%

90-99%

92-102%

 

General Annotation


Sub Unit:
N/A


Function:
Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.


Location:
Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Sample Data
Sample Data
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name: *
Email: *
Message: *
Inform
new Message
Package Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Package Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com