Home > ELISA Kit > Human IgE ELISA Kit

IgE (Shorten Name ),

Product Name:

Human IgE/ Immunoglobulin E ELISA Kit

Cat.#:

E0545h

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.78ng/mL

Specificity:

Natural and recombinant human Immunoglobulin E

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Immunology

Product Overview:

E0545h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin E) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin E. The ELISA analytical biochemical technique of the E0545h kit is based on Immunoglobulin E antibody-Immunoglobulin E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin E antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin E. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Si1ze & Price: Sign In.

Attention

Human IgE ELISA Kit

Email *

Message *

Please Sign In.

Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.5%

Recovery

Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	113	107-119
Plasma	115	109-120

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	101-112%	109-119%	86-96%	95-104%
EDTA plasma(n=5)	91-101%	108-118%	94-103%	86-99%
heparin plasma(n=5)	99-109%	101-113%	90-99%	92-102%

General Annotation

Sub Unit:

N/A

Function:

Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.

Location:

Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).

Related Products