027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Human IgE ELISA Kit
IgE (Shorten Name ),
Product Name:

Human IgE/ Immunoglobulin E ELISA Kit

Cat.#:

E0545h

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.78ng/mL
Specificity:
Natural and recombinant human Immunoglobulin E
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Human IgE ELISA Kit
Size & Price: Sign In.
×
Human IgE ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.5%

Recovery
Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

113

107-119

Plasma

115

109-120

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

101-112%

109-119%

86-96%

95-104%

EDTA plasma(n=5)

91-101%

108-118%

94-103%

86-99%

heparin plasma(n=5)

99-109%

 

101-113%

90-99%

92-102%

 

General Annotation


Sub Unit:
N/A


Function:
Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.


Location:
Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).


Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com