IgE (Shorten Name),
Product Name:
Human IgE/ Immunoglobulin E ELISA Kit
Cat.#:
E0545h
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.78ng/mL
Specificity:
Natural and recombinant human Immunoglobulin E
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Immunology
Product Overview:
E0545h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Immunoglobulin E) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Immunoglobulin E. The ELISA analytical biochemical
technique of the E0545h kit is based on Immunoglobulin E antibody-Immunoglobulin E antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Immunoglobulin E
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Immunoglobulin E. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤11.5%
Recovery
Recovery was determined by spiking various levels of Immunoglobulin E into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
113 |
107-119 |
|
Plasma |
115 |
109-120 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin E and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
101-112% |
109-119% |
86-96% |
95-104% |
|
EDTA plasma(n=5) |
91-101% |
108-118% |
94-103% |
86-99% |
|
heparin plasma(n=5) |
99-109%
|
101-113% |
90-99% |
92-102% |
General Annotation
Sub Unit:
N/A
Function:
Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are only 0.05% of the Ig concentration, compared to 10?mg/ml for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful inflammation reactions.
Location:
Immunoglobulin E (IgE) is a class of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is a monomeric antibody with 4 Ig-like domains (Cε1-Cε4).
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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