General CORTI/ Corticosterone ELISA Kit

Cat.#:

E0540Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

7.8-500ng/mL

Sensitivity:

1.9ng/mL

Specificity:

Natural and recombinant general Corticosterone

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0540Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Corticosterone) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Corticosterone. The ELISA analytical biochemical technique of the E0540Ge kit is based on Corticosterone antibody-Corticosterone antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Corticosterone antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Corticosterone. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General CORTI ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 4 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	84-93%	103-113%	94-103%
EDTA plasma(n=5)	91-91%	111-120%	96-106%	90-99%
heparin plasma(n=5)	111-121%	110-120%	107-116%	105-113%

General Annotation

Sub Unit:

N/A

Function:

In many species, including amphibians, reptiles, rodents and birds, corticosterone is a main glucocorticoid, involved in regulation of fuel, immune reactions, and stress responses. However, in humans, corticosterone is produced primarily in the zona fasciculata of the adrenal cortex. It has only weak glucocorticoid and mineralocorticoid potencies in humans and is important mainly as an intermediate in the steroidogenic pathway from pregnenolone to aldosterone. Corticosterone is converted to aldosterone by aldosterone synthase, found only in the mitochondria of glomerulosa cells. Glomerulosa cells are found in the Zona glomerulosa, which is the most superficial region of endocrine cells in the adrenal cortex.

Location:

Corticosterone (CORT) is a 21-carbon steroid hormone of the corticosteroid type produced in the cortex of the adrenal glands.

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