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CORTI (Shorten Name ),
Product Name:

General CORTI/ Corticosterone ELISA Kit

Cat.#:

E0540Ge

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
7.8-500ng/mL
Sensitivity:
1.9ng/mL
Specificity:
Natural and recombinant general Corticosterone
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
General CORTI ELISA Kit
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General CORTI ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Corticosterone and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

87-97%

84-93%

103-113%

94-103%

EDTA plasma(n=5)

91-91%

111-120%

96-106%

90-99%

heparin plasma(n=5)

111-121%

 

110-120%

107-116%

105-113%

 

General Annotation


Sub Unit:
N/A


Function:
In many species, including amphibians, reptiles, rodents and birds, corticosterone is a main glucocorticoid, involved in regulation of fuel, immune reactions, and stress responses. However, in humans, corticosterone is produced primarily in the zona fasciculata of the adrenal cortex. It has only weak glucocorticoid and mineralocorticoid potencies in humans and is important mainly as an intermediate in the steroidogenic pathway from pregnenolone to aldosterone. Corticosterone is converted to aldosterone by aldosterone synthase, found only in the mitochondria of glomerulosa cells. Glomerulosa cells are found in the Zona glomerulosa, which is the most superficial region of endocrine cells in the adrenal cortex.


Location:
Corticosterone (CORT) is a 21-carbon steroid hormone of the corticosteroid type produced in the cortex of the adrenal glands.


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