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PGE2 (Shorten Name),
Product Name:

General PGE2/ Prostaglandin E2 ELISA Kit

 
Cat.#:

E0538Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

dinoprostone

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
15.6-1000pg/mL
Sensitivity:
10pg/mL
Specificity:
Natural and recombinant general Prostaglandin E2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0538Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Prostaglandin E2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prostaglandin E2. The ELISA analytical biochemical technique of the E0538Ge kit is based on Prostaglandin E2 antibody-Prostaglandin E2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prostaglandin E2 antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Prostaglandin E2. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General PGE2 ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Prostaglandin E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

96-104%

94-103%

89-101%

105-115%

EDTA plasma(n=5)

99-109%

105-115%

108-118%

95-105%

heparin plasma(n=5)

90-102%

 

94-103%

94-104%

96-107%

 

General Annotation


Sub Unit:
N/A


Function:
The naturally occurring prostaglandin E2 (PGE2) is known in medicine as dinoprostone. It has important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. PGE2 is also the prostaglandin that ultimately induces fever.


Location:
Like other prostaglandins, dinoprostone can be used as an abortifacient. It is a direct vasodilator, relaxing smooth muscles, and it inhibits the release of noradrenaline from sympathetic nerve terminals. It does not inhibit platelet aggregation, where PGI2 does. It works by binding and activating the prostaglandin E2 receptor.


Sample Data
Sample Data
Sample Data
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