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NT-proBNP (Shorten Name),
Product Name:

Human NT-proBNP ELISA Kit

 
Cat.#:

E0485h

Brand:
EIAab®
Regulatory Status:
Alternative:

N-terminal prohormone of brain natriuretic peptide

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.12ng/mL
Specificity:
Natural and recombinant human NT-proBNP
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cardiovascular
Product Overview:
E0485h is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (NT-proBNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NT-proBNP. The ELISA analytical biochemical technique of the E0485h kit is based on NT-proBNP antibody-NT-proBNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NT-proBNP antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, NT-proBNP. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human NT-proBNP ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.8%

Recovery
Recovery was determined by spiking various levels of NT-proBNP into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-proBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-114%

88-96%

100-113%

114-124%

EDTA plasma(n=5)

111-120%

103-113%

106-116%

80-90%

heparin plasma(n=5)

96-106%

 

85-95%

99-109%

84-94%

 

General Annotation


Sub Unit:
N/A


Function:
The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) is a 76 amino acid N-terminal fragment of brain natriuretic peptide.


Location:
Both BNP and NT-proBNP levels in the blood are used for screening, diagnosis of acute congestive heart failure (CHF) and may be useful to establish prognosis in heart failure, as both markers are typically higher in patients with worse outcome. The plasma concentrations of both BNP and NT-proBNP are also typically increased in patients with asymptomatic or symptomatic left ventricular dysfunction.



[2].
Berta Esteban-Fernández de ávila , Vanessa Escamilla-Gómez , Susana Campuzano , María Pedrero , José M. Pingarrón,
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