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Cortisol (Shorten Name),
Product Name:

General Cortisol ELISA Kit

 
Cat.#:

E0462Ge

Brand:
EIAab®
Regulatory Status:
Alternative:

hydrocortisone

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.78-50ng/mL
Sensitivity:
0.31ng/mL
Specificity:
Natural and recombinant general Cortisol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0462Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Cortisol) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cortisol. The ELISA analytical biochemical technique of the E0462Ge kit is based on Cortisol antibody-Cortisol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cortisol antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Cortisol. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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General Cortisol ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cortisol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-106%

82-92%

108-118%

110-110%

EDTA plasma(n=5)

86-96%

109-119%

94-106%

86-96%

heparin plasma(n=5)

97-109%

 

83-93%

83-92%

108-118%

 

General Annotation


Sub Unit:
N/A


Function:
Cortisol (hydrocortisone) is a steroid hormone, more specifically a glucocorticoid, produced by the zona fasciculata of the adrenal gland. It is released in response to stress and a low level of blood glucocorticoids. Its primary functions are to increase blood sugar through gluconeogenesis; suppress the immune system; and aid in fat, protein and carbohydrate metabolism. It also decreases bone formation.


Location:
Cortisol is produced by the adrenal gland in the zona fasciculata, the second of three layers comprising the outer adrenal cortex. This release is controlled by the hypothalamus, a part of the brain. The secretion of corticotropin-releasing hormone (CRH) by the hypothalamus triggers anterior pituitary secretion of adrenocorticotropic hormone (ACTH). ACTH is carried by the blood to the adrenal cortex, where it triggers glucocorticoid secretion.


Sample Data
Sample Data
Sample Data
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