General E2/ Estradiol ELISA Kit

Cat.#:

E0461Ge

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

oestradiol,17β-estradiol

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

3.12-200ng/mL

Sensitivity:

1.33ng/mL

Specificity:

Natural and recombinant general Estradiol

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Product Overview:

E0461Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Estradiol) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Estradiol. The ELISA analytical biochemical technique of the E0461Ge kit is based on Estradiol antibody-Estradiol antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Estradiol antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Estradiol. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

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General E2 ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations ( 5 )
Sequence / 3D
Feedback Wall (0)

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estradiol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	95-104%	97-107%	102-112%
EDTA plasma(n=5)	104-114%	103-113%	104-113%	92-101%
heparin plasma(n=5)	89-99%	94-106%	87-97%	96-109%

General Annotation

Sub Unit:

N/A

Function:

Estradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3). Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect.

Location:

The serum levels of estradiol in males (14 - 55 pg/mL) are roughly comparable to those of postmenopausal women (< 35 pg/mL). Estradiol in vivo is interconvertible with estrone; estradiol to estrone conversion being favored. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs, including the bones.

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