E2 (Shorten Name ),
Product Name:
General E2/ Estradiol ELISA Kit
Cat.#:
E0461Ge
Brand:
EIAab®
Regulatory Status:
Alternative:
oestradiol, 17β-estradiol
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
3.12-200ng/mL
Sensitivity:
1.33ng/mL
Specificity:
Natural and recombinant general Estradiol
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations ( 5 )
- Sequence / 3D
- Feedback Wall (0)
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estradiol and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 87-97% | 95-104% | 97-107% | 102-112% |
EDTA plasma(n=5) | 104-114% | 103-113% | 104-113% | 92-101% |
heparin plasma(n=5) | 89-99%
| 94-106% | 87-97% | 96-109% |
General Annotation
Sub Unit:
N/A
Function:
Estradiol (E2 or 17β-estradiol, also oestradiol) is a sex hormone. Estradiol is abbreviated E2 as it has 2 hydroxyl groups in its molecular structure. Estrone has 1 (E1) and estriol has 3 (E3). Estradiol is about 10 times as potent as estrone and about 80 times as potent as estriol in its estrogenic effect.
Location:
The serum levels of estradiol in males (14 - 55 pg/mL) are roughly comparable to those of postmenopausal women (< 35 pg/mL). Estradiol in vivo is interconvertible with estrone; estradiol to estrone conversion being favored. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs, including the bones.
[2].
[3].
Nivin Sharawy, Mohamed Hassan, Laila Rashed, Wael Shawky, Moshira Rateb
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