DHEAS (Shorten Name),
Product Name:
General DHEAS/ DHEA sulfate ELISA Kit
Cat.#:
E0460Ge
Brand:
EIAab®
Regulatory Status:
Alternative:
Dehydroepiandrosterone sulfate
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.156-10ng/mL
Sensitivity:
0.054ng/mL
Specificity:
Natural and recombinant general DHEA sulfate
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
Product Overview:
E0460Ge is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (DHEA sulfate) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHEA sulfate. The ELISA analytical biochemical
technique of the E0460Ge kit is based on DHEA sulfate antibody-DHEA sulfate antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHEA sulfate
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, DHEA sulfate. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA sulfate and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
110-120% |
100-109% |
84-94% |
103-113% |
|
EDTA plasma(n=5) |
110-120% |
97-106% |
105-117% |
83-92% |
|
heparin plasma(n=5) |
113-123%
|
96-107% |
89-101% |
94-104% |
General Annotation
Sub Unit:
N/A
Function:
Dehydroepiandrosterone sulfate or DHEA sulfate is a metabolite of dehydroepiandrosterone (DHEA) produced by the addition of a sulfate group, catalyzed by the sulfotransferase enzymes SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.
Location:
N/A
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
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Publishing research using this product ?
Please let us know and we will provide a credit for your next order.
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Fax:
Email:sales@eiaab.com